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5 L Bioreactor for Microbial Fermentations

5 L Bioreactor for Microbial Fermentations

Novel magnetically coupled drive: eliminates the risk of contamination within the vessel since there is no mechanical seal.
Mixing and aeration: tailored for microbial fermentation (fungi, yeast or bacteria).
Autoclavable sampling port: The sterilisable port facilitates operational activities.
Uniform lid construction: No welded components within the lid for easy maintenance and sterility.
Flexible working volume: 5 L bioreactor with working volume up to 4.5 L

Product Description

Our innovative fully automated and autoclavable stirred tank bioreactor is designed for R&D and process development. It has a robust design, yet it is compact and ergonomic. The vessel is mounted between a jacketed base and a non-welded lid.

The uniform lid features all the necessary ports and sensors that might be required for bioprocesses.

There is a sterilisable sampling port conveniently located in the base of the vessel to facilitate operational activities during a process.

The magnetically coupled drive which can reach up to 1500 rpm ensures excellent sterility within the vessel.

 

The bioprocess controller ensures:

1) Temperature regulation by supplying a control signal either to the heating element or the electro-magnetic cooling water valve of the thermostat. Thermoregulation is carried out by circulation of

thermostated water through the bioreactor’s jacket, which is located in the bottom lid of the

vessel;

2) pH control by supplying base or acid solutions to the bioreactor medium using the

bioprocess controller’s peristaltic pumps;

3) pO2 control by automatic adjustments of the stirrer’s rotational speed. The actual pO2

value is monitored using a pO2 electrode;

4) Foam control by supplying an antifoam agent to the bioreactor’s medium using the

bioprocess controller’s peristaltic pumps. The foam level is monitored using a conductivity

sensor;

5) Feeding of a substrate by using the bioprocess controller’s peristaltic pump and the

respective feeding rate/volume is controlled by the feeding profile, which is set in the bioprocess

controller;

6) Level control by using the bioprocess controller’s peristaltic pump. The medium level is

monitored using a conductivity sensor;

7) Mixing by using a magnetic drive, the agitator is driven by a motor which is mounted on the top lid of the bioreactor.

Options

Bioreactor Product Option
5 L Bioreactor for Microbial Fermentation Optical density sensor with ARC view 265 transmitter and accessories (Hamilton)
Viable cell density sensor with ARC view 265 transmitter and accessories (Hamilton)
Methanol/ethanol gas sensor (BlueSens)
Methanol/ethanol immersed sensor (Raven Biotech)
Overpressure sensor , autoclavable, 0-1 bar, measuring accuracy +/- 0.02 bar
Overpressure control, 0-1 bar, control accuracy +/- 0.0 bar.
O₂/CO₂ analyzer BlueInOne Ferm (BlueSens), including flow adapter
Mass flow controller for air
Mass flow controller for O₂
Volumetric oxygen mass transfer coefficient kLa, Oxygen uptake rate OUR, CO₂ exchange rate CER (includes overpressure sensor and O₂/CO₂ analyzer)
Pitched blade impeller set with bushings
Rushton turbine set with bushings
Microsparger
Factory accepted test FAT
SCADA for monitoring, control and reporting according the requirements of 21 CFR Part 11

Specification

Bioreactor 5.1 (microbiology)
Vessel
Total Volume (L) 6.2
Working Volume (L) 2 – 4.5
Inside Diameter/Inside Height (mm) 150 / 350
Ports Mixer drive; pH, DO, T, foam, liquid level sensors; tree needle port for acid base and anti-foam agent addition; chemo-stat tubes for level control and addition of bioreactor feed solutions; aseptic pierce-able membrane port; in-let gas port (0.2 μm filter added); exhaust gas port (0.2 μm filter added); 2 spare ports/ The ports are 4×10 mm, 4 x 7.5 mm –7×12 mm (PG 13.5 mm)
Sampling Double walled base ensuring bioreactor thermostating. Autoclavable aseptic sampler
Aeration
Control Rotameters (2), TMFC (option)
Gas Supply Air and oxygen
Flow Range, L/min 0.5 -8.6
Sparger Ring
Filters D50 mm 0.2 µm PTFE
Exhaust gas condenser Cooling from water – line or chiller
Mixing
Drive Top magnetic coupling
Rotation Speed Range (rpm) 40 – 1300
Mixer (Impellers) 2 rushton turbines
Control
Controller Siemens Simatic S7 – 1500
Operator panel Touch screen Beetronics, 15TS7, 15’’
Temperature Built-in thermostat. Control range: from 5°С via coolant to 60°С. Accuracy:(measurement) +/- 0.1°C, (control) +/- 0.2°C
pH Hamilton sensors (different options, including ARC). Acid/ Base 2 – 12 +/- 0,01pH units
pO₂ Hamilton sensors
Foam Conductivity sensor
Overpressure (option) The control range 0-1 bar, measuring accuracy +/- 0.02 bar, control accuracy +/- 0.04 bar
Feeding 0.02 – 40 ml/min according to adjusted profile
Peristaltic Pumps 4 built-in peristaltic pumps. External pump (option)
Communication and data exchange Ethernet connection to LAN or WAN for remote maintenance or OPC; WiFi connection (VNC server) for smart phones and tablets
Dimensions (mm) 930 (W) х 800(H) х 600(D)
Autoclaving space required (mm) 590 (H) x 270 (D)
Optional sensors Culture turbidity (optical density), culture permittivity (viable cell density), conductivity, methanol/ethanol, off-gas analysis (Oxygen, Carbon dioxide, CH₄), etc.
Fed-batch control (option) Model based fed-batch control, using supplement PC program (based on Matlab or Python), and connected with SCADA trough OPC server.

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