15L Bioreactor for Microbial Fermentations
Novel magnetically coupled drive: eliminates the risk of contamination within the vessel since there is no mechanical seal.
Mixing and aeration: tailored for microorganisms (fungi, yeast or bacteria).
Autoclavable sampling port: The sterilisable port facilitates operational activities.
Uniform lid construction: No welded components within the lid for easy maintenance and sterility.
Flexible working volume: 15 L bioreactor with working volume up to 11 L.
Our 15 L stirred tank bioreactor is designed for microbial fermentations though may be adapted for cell cultivation. The autoclavable microbial bioreactor is ideal for use within R&D and process development. The borosilicate glass vessel is mounted between a jacketed base and a non-welded lid.
The uniform lid features all the necessary ports and sensors that might be required for bioprocesses and features a convenient carrying handle. Featuring twelve ports comprising of five ports for sensor connections, three ports for bioreactor’s liquid supply, two ports for gas sparger and gas output and two spare ports that can be user defined.
There is a sterilisable sampling port conveniently located in the base of the vessel to facilitate operational activities during a process. The overall design of the reactor is simple to maintain and apply basic operations and processes.
The magnetically coupled drive which can reach up to 700 rpm ensures excellent sterility within the vessel throughout fermentation.
The bioreactor process control ensures:
Temperature (the temperature sensor (Pt100) is located in the bioreactor tank) regulation. Carried out by supplying a control signal either to the heating element or the electro-magnetic cooling water valve of the thermostat. Thermoregulation is carried out by circulation of thermostated water through the bioreactor’s jacket, which is located in the bottom lid of the vessel;
pH control. Carried out by supplying base or acid solutions to the bioreactors medium using the bioprocess controller’s peristaltic pumps;
pO2 control. Ensured by automatic adjustments of the stirrer’s rotational speed. The actual pO2 value is monitored using a pO2 electrode;
Foam control. Carried out by supplying an antifoam agent to the bioreactor’s medium using the bioprocess controller’s peristaltic pumps. The foam level is monitored using a conductivity sensor;
Feeding (of a substrate). Carried out using the bioprocess controller’s peristaltic pump and the respective feeding rate/volume is controlled by the feeding profile, which is set in the bioprocess controller;
6) Level control. Carried out using the bioprocess controller’s peristaltic pump. The medium level is monitored using a conductivity sensor;
7) Mixing. Ensured by a magnetic drive (stirrer inner shaft and impellers contain magnets), the agitator is driven by a motor which is mounted on the top lid of the bioreactor.
Optional SCADA software is available to control fermentation processes and store the process data. Additional sensors, balances and devices can be integrated in SCADA. The software can operate on a single PC, network or remotely via Internet and can control one or several bioreactors.
Our SCADA enables the possibility to communicate with MATLAB based program via OPC server. The MATLAB program ensures fed-batch automatic control by on-line comparison of mathematical model with process off-line data.
The SCADA software is developed according to the requirements of 21 CFR Part 11. The implication is that the process can be evaluated, reviewed, approved and archived without a single sheet of paper and without the possibility to falsify process data and signatures. Our SCADA software may be adapted to the special requirements of our customers upon request.
|Bioreactor 15.1 (microbiology)|
|Total Volume (L)||16|
|Working Volume (L)||5 – 11|
|Inside Diameter/Inside Height (mm)||200 / 500|
|Ports||For mixer/agitator shaft; for pH, dissolved oxygen, temperature, foam, and liquid level sensors; tree needle port for acid base and anti-foam agent addition; chemo-stat tubes for level control and addition of bioreactor feed solutions; aseptic pierce-able membrane port; in-let gas port (0.2 μm filter added); exhaust gas port (0.2 μm filter added); 2 spare ports/ The ports are 4×10 mm, 4 x 7.5 mm – 7×12 mm (PG 13.5 mm)|
|Sampling||Double walled base ensuring bioreactor thermostating. Autoclavable aseptic sampler|
|Control||Rotameters (2), TMFC (optional)|
|Gas Supply||Air and oxygen|
|Flow Range, L/min||0.5 – 20 (Air) or 0 – 10 (O₂)|
|Filters||D50 mm 0.2 μm PTFE|
|Exhaust gas condenser||Cooling from water line or chiller|
|Drive||Top magnetic coupling|
|Rotation Speed Range (rpm)||40 – 700|
|Mixer (Impellers)||3 standard Rushton turbines|
|Controller||Siemens Simatic S7 – 1500|
|Operator panel||Touch screen Beetronics, 15TS7, 15’’|
|Temperature||Control limits: from 5°C above cooling water up to 60 °C. Measurement accuracy +/- 0.1 °C and control accuracy +/- 0.2 °C. Built-in thermostat|
|pH|| Hamilton sensors (different options, including ARC). Acid/ Base
2 – 12 +/- 0.01pH units
|pO₂||Hamilton sensor. (different options, including Arc). Control: mixing + О₂ +feeding
0 – 150 % +/- 1%
|Feeding||0.02-40 ml/min according to the set profile|
|Peristaltic Pumps||4 built-in peristaltic pumps. External pump (option)|
|Dimensions (mm)||Overall dimensions of the bioreactor (mm) 1000 (W) х 970 (H) х 600 (D)
The minimum size of the autoclave chamber for vessel autoclaving (mm)
760 (Height) х 320 (Diameter))
|15 L Microbial Bioreactor||Optical density sensor with ARC view 265 transmitter and accessories (Hamilton)|
|Viable cell density sensor with ARC view 265 transmitter and accessories (Hamilton)|
|Methanol/ethanol gas sensor (BlueSens)|
|Methanol/ethanol immersed sensor (Raven Biotech)|
|Overpressure sensor, autoclavable, 0-1 bar, measuring accuracy +/- 0.02 bar|
|Overpressure control, 0-1 bar, control accuracy +/- 0.0 bar.|
|O₂/CO₂ analyzer BlueInOne Ferm (BlueSens), including flow adapter|
|Mass flow controller for air|
|Mass flow controller for O₂|
|Volumetric oxygen mass transfer coefficient kLa, Oxygen uptake rate OUR, CO₂ exchange rate CER (includes overpressure sensor and O₂/CO₂ analyzer)|
|Rushton turbine set with bushings|
|Factory accepted test FAT|
|SCADA for monitoring, control and reporting according the requirements of 21 CFR Part 11|