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1 L Twin Microbial Bioreactor

1 L Twin Microbial Bioreactor

Novel magnetically coupled drive: eliminates the risk of contamination within the Bioreactor since as there is no mechanical seal.
Powerful Peltier temperature control: The Peltier element provides accurate temperature control from 10-50°C.
Mixing and aeration: tailored for microbial fermentation (fungi, yeast or bacteria).
Uniform lid construction: No welded components within the lid for easy maintenance and sterility.
Flexible working volume: 1 L bioreactor with working volume of 0.4 – 0.7 L.

Product Description

Our innovative twin stirred tank bioreactor is designed for R&D and process development. It has a robust design, yet it is compact and ergonomic.

A Peltier element efficiently controls the temperature of the bioreactor vessel without the need for a water source. Tough construction and fan-driven heat exchange develop sufficient power to cool and condense even high-temperature processes.

The uniform non-welded lid features all the necessary ports and sensors that might be required for bioprocesses.

The magnetically coupled drive ensures excellent sterility within the vessel since it eliminates the need for a mechanical seal. It can provide a mixing rate from 40 rpm to 1000 rpm.

The bioprocess control unit ensures:

1) Temperature moderation. Temperature is controlled by a thermoelectric heating and cooling element (Peltier element) and is measured with the Pt100 sensor. Thermoregulation of the vessels is performed by placing the base of the vessels in copper heat transfer jars;

2) pH control. Enabled by supplying base or acid solutions to the bioreactors medium using the control unit’s peristaltic pumps;

3) pO2 control. Ensured by automatic adjustments of the stirrer’s rotational speed. The actual pO2 value is monitored using a pO2 electrode;

4) Foam control. Performed by supplying an antifoam agent to the bioreactor’s medium using the control unit’s peristaltic pumps. The foam level is monitored using a conductivity sensor;

5) Feeding (of a substrate). Carried out using the control unit’s peristaltic pump and the respective feeding rate/volume is controlled by the feeding profile, which is set in the control unit;

6) Mixing. Ensured by a magnetic drive, the agitator is driven by a motor which is mounted on the top lid of the bioreactor.

Options

Bioreactor Product Option
1 L Twin Bioreactor for Microbial Fermentation O2/CO2 analyzer BlueInOne Ferm (BlueSens), including flow adapter
Mass flow controller for air
Mass flow controller for O2
Volumetric oxygen mass transfer coefficient kLa, Oxygen uptake rate OUR, CO2 exchange rate CER (includes overpressure sensor and O2/CO2 analyzer)
Microsparger
Factory accepted test FAT
SCADA for monitoring, control and reporting according the requirements of 21 CFR Part 11

Specification

Bioreactor 1.1 (microbiology)  
Vessel
Total Volume (L) 0.9
Working Volume (L) 0.4 – 0.7
Inside Diameter/Inside Height (mm) 85 / 165
Ports Mixer drive; pH, DO, T, foam sensors; 2 medium inlet/outlet; exhaust gas, sparger
Aeration
Control Rotameter, TMFC (optional)
Gas Supply Air, O₂ (optional)
Flow Range, L/min 0.1-2.0
Sparger Micro sparger
Filters D37 mm 0.2 μm PTFE
Exhaust gas condenser Peltier cooling
Mixing
Drive Top, magnetic coupling
Rotation Speed Range (rpm) 40-1000
Mixer (Impellers) 2 Rushton turbines
Control
Controller Siemens Simatic S7-1200
Operator panel Touch screen 12”
Temperature Peltier heating/cooling 10-50°C. Measurement accuracy ± 0.1°C and control accuracy ± 0.2°C
pH Hamilton or Mettler Toledo sensor. Acid/Alkali 2-12 +/- 0.01 pH units
pO₂ Hamilton or Mettler Toledo sensor. Stir control + О₂ (optional). 0-150% +/- 1%
Foam Conductivity sensor
Feeding 0.01-20 mL/min according to the set profile
Peristaltic Pumps 3 built-in variable speed pumps. Additional pumps (optional)
Dimensions Overall dimensions of the bioreactor (mm) 540(W) х 350(H) х 410(D)

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