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5 L Bioreactor for Cell Cultivations

5 L Bioreactor for Cell Cultivations

Novel magnetically coupled drive: eliminates the risk of contamination within the vessel since there is no mechanical seal.
Mixing and aeration: tailored for cell cultivation (plant and mammalian cells).
Autoclavable sampling port: The sterilisable port facilitates operational activities.
Uniform lid construction: No welded components within the lid for easy maintenance and sterility.
Flexible working volume: 5 L bioreactor with working volume up to 4.5 L.

Descripción

Our innovative fully automated and autoclavable laboratory bioreactor is designed for R&D and process development. It has a robust design, yet it is compact and ergonomic. The vessel is mounted between a jacketed base and a non-welded lid.

The uniform lid features all the necessary ports and sensors that might be required for cell cultivation processes.

There is a sterilisable sampling port conveniently located in the base of the vessel to facilitate operational activities during a process.

The magnetically coupled drive which can reach up to 1500 rpm ensures excellent sterility within the vessel.

 

The bioprocess controller ensures:

1) Temperature regulation by supplying a control signal either to the heating element or the electro-magnetic cooling water valve of the thermostat. Thermoregulation is carried out by circulation of thermostated water through the bioreactor’s jacket, which is located in the bottom lid of the vessel;

2) pH control by supplying base or acid solutions to the bioreactor medium using the bioprocess controller’s peristaltic pumps;

3) pO2 control by automatic adjustments of the stirrer’s rotational speed. The actual pO2 value is monitored using a pO2 electrode;

4) Foam control by supplying an antifoam agent to the bioreactor’s medium using the bioprocess controller’s peristaltic pumps. The foam level is monitored using a conductivity sensor;

5) Feeding of a substrate by using the bioprocess controller’s peristaltic pump and the respective feeding rate/volume is controlled by the feeding profile, which is set in the bioprocess

controller;

6) Level control by using the bioprocess controller’s peristaltic pump. The medium level is monitored using a conductivity sensor;

7) Mixing by using a magnetic drive, the agitator is driven by a motor which is mounted on the top lid of the bioreactor.

Specification

Bioreactor 5.2 (cell cultures)
Vessel
Total Volume (L) 6.2
Working Volume (L) 2 – 4.5
Inside Diameter/Inside Height (mm) 150 / 350
Ports Mixer drive; pH, DO, T, foam, liquid level sensors; tree needle port for acid base and anti-foam agent addition; chemo-stat tubes for level control and addition of bioreactor feed solutions; aseptic pierce-able membrane port; in-let gas
port (0.2 μm filter added); exhaust gas port (0.2 μm filter added); 2 spare ports/ The ports are 4×10 mm, 4 x 7.5 mm – 7×12 mm (PG 13.5 mm)
Sampling Double walled base ensuring bioreactor thermostating. Autoclavable aseptic sampler
Aeration
Control Rotameters (2), TMFC (option)
Gas Supply Air/Carbon dioxide + Oxygen, N₂ (option) Air/Carbon dioxide in headspace
Flow Range, L/min 0.1 – 2
Sparger Microsparger
Filters D50 mm 0.2 µm PTFE
Exhaust gas condenser Cooling from water – line or chiller
Mixing
Drive Top magnetic coupling
Rotation Speed Range (rpm) 40 – 500
Mixer (Impellers) 2 pitched blade impellers
Control
Controller Siemens Simatic S7 – 1500
Operator panel Touch screen Beetronics, 15TS7, 15’’
Temperature Built-in thermostat. Control range: from 5°С via coolant to 60°С. Accuracy:(measurement) +/- 0.1°C, (control) +/- 0.2°C
pH Hamilton sensors (different options, including ARC). Acid/ Base Base/СО2 2 – 12 +/- 0,01pH units
pO₂ Hamilton sensors (different options, including Arc).
Foam Option
Overpressure (option) The control range 0-1 bar, measuring accuracy +/- 0.02 bar, control accuracy +/- 0.04 bar
Feeding 0.02 – 40 ml/min according to adjusted profile
Peristaltic Pumps 4 built-in peristaltic pumps. External pump (option)
Communication and data exchange Ethernet connection to LAN or WAN for remote maintenance or OPC; WiFi connection (VNC server) for smart phones and tablets
Dimensions (mm) 930 (W) х 800(H) х 600(D)
Autoclaving space required (mm) 590 (H) x 270 (D)
Optional sensors Culture turbidity (optical density), culture permittivity (viable cell density), conductivity, methanol/ethanol, off-gas analysis (Oxygen, Carbon dioxide, CH₄), etc.
Fed-batch control (option) Model based fed-batch control, using supplement PC program (based on Matlab or Python), and connected with SCADA trough OPC server.

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